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" Mycotoxin protocols / "
edited by Mary W. Trucksess and Albert E. Pohland.
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BL
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| Record Number
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953749
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b708119
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| Title & Author
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Mycotoxin protocols /\ edited by Mary W. Trucksess and Albert E. Pohland.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©2001.
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| Series Statement
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Methods in molecular biology ;; 157
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| Page. NO
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1 online resource (xii, 244 pages) :: illustrations
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| ISBN
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0896036235
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: 1280820551
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: 1592590640
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: 6610820554
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: 9780896036239
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: 9781280820557
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: 9781592590643
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: 9786610820559
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9781489938725
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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pt. 1. General techniques -- Mycotoxin method evaluation: an introduction -- Sampling techniques -- Preparatory isolation of mycotoxins from solid phase fungal cultures -- Preparation of mycotoxin standards -- Electrospray mass spectrometry for mycotoxin detection and purity analysis -- pt. 2. Methods for aspergillus toxins -- Measurement of aflatoxins using capillary electrophoresis -- Immunochemical method for cyclopiazonic acid -- Immunochemical method for ochratoxin A -- pt. 3. Methods for fusarium toxins -- Solution fluorometric method for deoxynivalenol in grains -- Chromatographic method for trichothecenes -- Chromatographic method for the determination of the mycotoxin moniliformin in corn -- Liquid chromatographic method for fumonsins in corn -- Enzyme-linked immunosorbent assays of zearalenone using polyclonal, monoclonal, and recombinant antibiodies -- pt. 4. Methods for other fungal toxins -- Chromatographic method for stachybotrys toxins -- Immunochemical method for citrinin -- Solid phase extraction method for patulin in apple juide and unfiltered apple juice -- Liquid chromatographic method for the determination of ergot alkaloids in cereal grains -- Chromatographic method for alternaria toxins in apple juice -- Index.
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| Abstract
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Mycotoxins, common food contaminants produced by molds, are associated with a broad range of serious toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity, are subject to regulatory restrictions in more than 80 countries. In Mycotoxin Protocols, a panel of accomplished scientists describe their innovative, cutting-edge methods for determining the levels of various mycotoxins in foods and feeds. Almost half of the methods presented involve molecular-based immunochemical or immunochemical/chromatographic techniques. The necessary equipment, reagents, and procedures are given in great detail for the analysis of a wide variety of significant mycotoxins, including aflatoxins, aflatoxin M1, cyclopiazonic acid, ochratoxin A, trichothcenes, moniliformin, fumonisins, zearalenone, Stachybotrys toxins, citrinin, patulin, ergot alkaloids, and Alternaria toxins. General techniques for mycotoxin analyses, sampling procedures for collecting representative test samples, isolation techniques, and techniques for the detection and identification of toxins and impurities are also included. Up-to-date and highly practical, Mycotoxin Protocols provides a comprehensive collection of the latest bioanalytical techniques for determining mycotoxins in foods and feeds.
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| Subject
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Mycotoxins, Laboratory manuals.
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MEDICAL-- Toxicology.
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Mycotoxins.
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Mycotoxins-- analysis.
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Wetenschappelijke technieken.
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| Dewey Classification
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615.9/5295
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| LC Classification
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QP632.M9M895 2001eb
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| NLM classification
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QW 630.5.M9M9945 2001
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W1ME9616J v.157 2001
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| Added Entry
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Pohland, Albert E.
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Trucksess, Mary W.
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