| Document Type
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BL
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| Record Number
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958617
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b712987
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| Title & Author
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Free radical and antioxidant protocols /\ edited by Donald Armstrong.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©1998.
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| Series Statement
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Methods in molecular biology ;; v. 108
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| Page. NO
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1 online resource (xvii, 455 pages) :: illustrations
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| ISBN
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058523566X
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: 0896034720
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: 1280835982
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: 1592592546
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: 6610835985
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: 9780585235660
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: 9780896034723
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: 9781280835988
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: 9781592592548
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: 9786610835980
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Oxygen consumption methods. Xanthine oxidase and lipoxygenase / I. Stadler -- Spin trapping and electron paramagnetic resonance spectroscopy / D.J. Brackett [and others] -- In vivo detection of free radicals in real time by low-frequency electron paramagnetic resonance spectroscopy / G.M. Rosen, S. Pou and H.J. Halpern -- Single photon counting / Y. Mizuguchi -- Measurement of reactive oxygen species in whole blood and mononuclear cells using chemiluminescence / K. Thusu, E. Abdel-Rahman and P. Dandona -- Assay of phospholipid hydroperoxides by chemiluminescence-based high-performance liquid chromatography / Y. Yamamoto, Y. Kambayashi and T. Ueda -- Sensitive and specific fluorescent probing of oxidative stress in different classes of membrane phospholipids in live cells using metabolically integrated CIS-parinaric acid / V.E. Kagan [and others] -- Aromatic hydroxylation. Salicylic acid as a probe for measuring hydroxyl radical production / A. Ghiselli -- Simple assay for the level of total lipid peroxides in serum or plasma / K. Yagi -- Simple procedure for specific assay of lipid hydroperoxides in serum or plasma / K. Yagi -- Antibodies against malondialdehyde-modified proteins. Induction and elisa measurement of specific antibodies / Y. Chancerelle, J. Mathieu and J.F. Kergonou -- Oxidized ldl and lp(a). Preparation, modification, and analysis / J. Galle and C. Wanner -- Oxidized and unoxidized fatty acyl esters / O. Sergent and J. Cillard -- Synthesis of lipid and cholesterol hydroperoxide standards / D. Armstrong and R.W. Browne -- Separation of hydroxy and hydroperoxy polyunsaturated fatty acids by high-pressure liquid chromatography / R.W. Browne and D. Armstrong -- Products of creatinine with hydroxyl radical as a useful marker of oxidative stress in vivo / K. Aoyagi [and others] -- Quantitative analysis of peptide and protein changes in ischemic hippocampal tissue by HPLC / T.M. Wengenack, J.R. Slemmon and J.M. Ordy -- Electrochemical detection of 8-hydroxy-2-deoxyguanosine levels in cellular DNA / T.M. Nicotera and S. Bardin -- Nitric oxide synthase / A. Aljada and P. Dandona -- Quantitation of heme oxygenase (ho-1) copies in human tissues by competitive RT/PCR / N.G. Abraham -- A fluorescent-based assay for measurement of phospholipase a2 activity. A facile assay for cell sonicates / T.M. Nicotera and G. Spehar -- Autofluorescent ceroid/lipofuscin / D. Yin and U. Brunk -- In vivo technique for autofluorescent lipopigments / F.C. Delori and C.K. Dorey -- An ex vivo erythrocyte model for investigating oxidative metabolism / L. Rossi and M. Magnani -- Purification of vesicular carriers from rat hepatocytes by magnetic immunoadsorption / L. Saucan -- Simultaneous determination of serum retinol, tocopherols, and carotenoids by HPLC / R.W. Browne and D. Armstrong -- EPR measurements of nitric oxide-induced chromanoxyl radicals of vitamin e. Interactions with vitamin c / V.E. Kagan and N.V. Gorbunov -- Nonvitamin plasma antioxidants / N.J. Miller -- Regulatory antioxidant enzymes / C.E. Pippenger, R.W. Browne and D. Armstrong -- In vitro screening for antioxidant activity / D. Armstrong, T. Hiramitsu and T. Ueda -- Antioxidant activity of low-density lipoprotein / N.J. Miller and G. Paganga -- Lipoic acid as an antioxidant. The role of dihydrolipoamide dehydrogenase / M.S. Patel and Y.S. Hong -- Reduced glutathione and glutathione disulfide / R.W. Browne and D. Armstrong -- Analysis of coenzyme Q10 content in human plasma and other biological samples / S. Graves [and others] -- A simple luminescence method for detecting lipid peroxidation and antioxidant activity in vitro / M. Nakano, T. Ito and T. Hiramitsu -- Analysis of oxidation and antioxidants using microtiter plates / G. Camejo, B. Wallin and M. Enojarvi -- Trace element analysis of biological samples by analytical atomic spectroscopy / H. Haraguchi, E. Fujimori and K. Inagaki -- Energy dispersive X-ray microanalysis / D.A. Samuelson -- Pro-oxidant and antioxidant effects of estrogens / J.G. Liehr and D. Roy -- Artificial radical generating and scavenging systems. Photo-fenton reagent and caged compounds / S. Matsugo and K. Fujimori -- Free radical and antioxidant protocols.
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| Abstract
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In Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. Free Radical and Antioxidant Protocols provides state-of-the-art methodology and biotechnology in a convenient format for both academic and corporate biomedical scientists. The detailed, laboratory-tested free-radical assays, many of them presented here for the first time, will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.
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| Subject
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Antioxidants-- Physiological effect, Laboratory manuals.
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Free radicals (Chemistry)-- Pathophysiology, Laboratory manuals.
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Oxidation, Physiological, Laboratory manuals.
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Stress (Physiology)-- Research, Laboratory manuals.
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Antioxidants-- metabolism.
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Free Radicals-- metabolism.
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Oxidative Stress-- physiology.
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Antioxydants-- Effets physiologiques-- Manuels de laboratoire.
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Oxydation biologique-- Manuels de laboratoire.
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Radicaux libres (Chimie)-- Physiopathologie-- Manuels de laboratoire.
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Stress-- Recherche-- Manuels de laboratoire.
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Antioxidants-- Physiological effect.
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Free radicals (Chemistry)-- Pathophysiology.
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MEDICAL-- Pathology.
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MEDICAL-- Pathophysiology.
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Oxidation, Physiological.
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Stress (Physiology)-- Research.
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Antioxidanten.
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Radicalen (chemie)
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Wetenschappelijke technieken.
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| Dewey Classification
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616.07
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| LC Classification
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RB170.F68 1998eb
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| NLM classification
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QU 25F853 1998
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W1ME9616J v.108 1998
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44.33bcl
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| Added Entry
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Armstrong, Donald,1931-
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