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" Peptide nucleic acids : "
edited by Peter E. Nielsen.
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BL
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| Record Number
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958683
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b713053
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| Title & Author
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Peptide nucleic acids : : methods and protocols /\ edited by Peter E. Nielsen.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©2002.
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| Series Statement
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Methods in molecular biology ;; v. 208
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| Page. NO
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1 online resource (x, 274 pages) :: illustrations
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| ISBN
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0896039765
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: 1280830743
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: 1592592902
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: 6610830746
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: 9780896039766
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: 9781280830747
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: 9781592592906
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: 9786610830749
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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PNA technology / Peter E. Nielsen -- Solid phase synthesis of PNA oligomers / Frederik Beck -- Synthesis of PNA-peptide conjugates / Satish Kumar Awasthi -- Parallel synthesis of PNA-peptide conjugate libraries / Satish Kumar Awasthi -- Thermodynamics of PNA interactions with DNA and RNA / Tommi Ratilainen -- Measurement of PNA binding to double-stranded DNA / Thomas Bentin -- PNA-mediated immobilization of supercoiled DNA / Thomas Bentin -- PNA openers and their applications /Vadim V. Demidov -- Colorimetric detection of PNA-DNA hybridization using cyanine dyes / Miaomiao Wang -- PNA-medicated PCR clamping: applications and methods / Deborah G. Murdock -- PNA clamping techniques for the determination of oncogene mutations / Marcus Schuermann -- PNA fluorescent in situ hybridization for rapid microbiology and cytogenetic analysis / Brett Williams -- Detection of point mutations using PNA-containing electrophoresis matrices / Gabor L. Igloi -- Lipid-mediated introduction of peptide nucleic acids into cells / Dwaine A. Braasch -- Synthesis of cell-penetrating peptide-PNA constructs / Margus Pooga -- Antisense inhibition of bacterial gene expression and cell growth / Liam Good -- In vitro transcription from peptide nucleic acid/DNA strand displacement loops / Niels Erik Mollegaard -- In vitro and in vivo studies on pharmacokinetics and metabolism of PNA constructs in rodents / Edward Kristensen.
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| Abstract
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Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on experience in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process. Up-to-date and highly practical, Peptide Nucleic Acids: Methods and Protocols provides for both novice and experienced researchers a thorough and easy introduction to the optimal use of today's PNA technology across a wide variety of fields, including molecular biology, medicinal chemistry, genetic diagnostics, and drug development.
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DNA-- Derivatives.
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Peptides-- Derivatives.
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ADN-- Dérivés.
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Peptides-- Dérivés.
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DNA-- Derivatives.
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SCIENCE-- Life Sciences-- Molecular Biology.
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Peptide Nucleic Acids.
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Nucleïnezuren.
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Peptiden.
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Wetenschappelijke technieken.
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| Dewey Classification
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572.8
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| LC Classification
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QP624.P47 2002
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| NLM classification
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QU 58P4239 2002
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W1ME9616J v.208 2002
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| Added Entry
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Nielsen, Peter E.,1951-
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