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" Bioluminescence : "
edited by Preston B. Rich and Christelle Douillet.
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BL
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| Record Number
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960063
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b714433
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| Title & Author
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Bioluminescence : : methods and protocols /\ edited by Preston B. Rich and Christelle Douillet.
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| Edition Statement
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2nd ed.
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| Publication Statement
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Dordrecht ;New York :: Humana Press/Springer,, ©2009.
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| Series Statement
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Methods in molecular biology,; 574
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Springer protocols
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| Page. NO
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1 online resource (xi, 267 pages) :: illustrations (some color)
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| ISBN
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1603273212
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: 9781603273213
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1603273204
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9781603273206
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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1. Luminescent probes and visualization of bioluminescence / Elisa Michelini [and others] -- 2. Validation of bioluminescent imaging techniques / John Virostko and E. Duco Jansen -- 3. Assessment of extracellular ATP concentrations / Lucia Seminario--Vidal, Eduardo R. Lazarowski, and Seiko F. Okada -- 4. High-throughput quantitative bioluminescence imaging for assessing tumor burden / Angelina Contero [and others] -- 5. Fluorescence imaging of tumors with "smart" pH-activatable targeted probes / Daisuke Asanuma [and others] -- 6. Imaging vasculature and lymphatic flow in mice using quantum dots / Byron Ballou [and others] -- 7. Bioluminescent imaging of transplanted islets / Xiaojuan Chen and Dixon B. Kaufman -- 8. Bioluminescence reporter gene imaging of human embryonic stem cell survival, proliferation, and fate / Kitchener D. Wilson, Mei Huang, and Joseph C. Wu -- 9. Detection of apoptosis using cyclic luciferase in living mammals / Akira Kanno, Yoshio Umezawa, and Takeaki Ozawa -- 10. Noninvasive bioluminescent imaging of infections / Javier S. Burgos -- 11. Real-time bioluminescence imaging of viral pathogenesis / Kathryn E. Luker and Gary D. Luker -- 12. Bioluminescent monitoring of in vivo colonization and clearance dynamics by light-emitting bacteria / Siouxsie Wiles [and others] -- 13. Quantitative in vivo imaging of non-viral-mediated gene expression and RNAi-mediated knockdown / Garrett R. Rettig and Kevin G. Rice -- 14. Analysis of protein-protein interactions using bioluminescence resonance energy transfer / Kevin D.G. Pfleger -- 15. Bioluminescent imaging of MAPK function with intein-mediated reporter gene assay / Akira Kanno, Takeaki Ozawa, and Yoshio Umezawa -- 16. Bioluminescence analysis of Smad-dependent TGF-b signaling in live mice / Jian Luo and Tony Wyss-Coray -- 17. Bioluminescence imaging of calcium oscillations inside intracellular organelles / Carlos Villalobos, Marıa Teresa Alonso, and Javier Garcia-Sancho -- 18. Novel tools for use in bioluminescence resonance energy transfer (BRET) assays / Mélanie Robitaille [and others] -- 19. PIN-G reporter for imaging and defining trafficking signals in membrane proteins / Lynn Mckeown, Vicky C. Jones, and Owen T. Jones -- 20. Imaging b-galactosidase activity in vivo using sequential reporter-enzyme luminescence / Georges von Degenfeld, Tom S. Wehrman, and Helen M. Blu.
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| Abstract
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Through the study and application of bioluminescence, scientists have painstakingly harnessed a powerful tool that enables us to seek a deeper understanding of the complex mechanisms underpinning so many vital biologic systems. In this fully revised and updated second edition of Bioluminescence: Methods and Protocols, expert researchers contribute a readable and utilitarian compilation of the newest and most innovative techniques that have emerged in this rapidly expanding and progressively diverse field including methods to assess cell trafficking, protein-protein interactions, intracellular signaling, and apoptosis. Also opening up the possibility to visualize and quantify biological mechanisms in real-time and in in vivo settings, the volume also describes the in vivo study of bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials, equipment, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioluminescence: Methods and Protocols, Second Edition provides protocols that are detailed enough to be followed and adapted by scientific teams who have no previous expertise in bioluminescence in order to foster the potentially numerous breakthroughs and new applications from basic to applied science and medicine that must continue to be developed.
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| Subject
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Bioluminescence assay.
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Bioluminescence assay.
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Luminescent Measurements.
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Biosensing Techniques.
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Laboratory Techniques and Procedures
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| Dewey Classification
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572/.36
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| LC Classification
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QP519.9.B55B536 2009
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| NLM classification
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QP 519.9.B55
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W1ME9616J v.574 2009
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| Added Entry
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Douillet, Christelle.
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Rich, Preston B.
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