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" Retinoids : "
edited by Hui Sun, Gabriel H. Travis.
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BL
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| Record Number
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960403
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b714773
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| Title & Author
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Retinoids : : methods and protocols /\ edited by Hui Sun, Gabriel H. Travis.
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| Publication Statement
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[Totowa, N.J.] :: Humana Press,, ©2010.
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| Series Statement
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Methods in molecular biology,; 652
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Springer protocols
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| Page. NO
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1 online resource (x, 365 pages) :: illustrations (some color)
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| ISBN
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1603273255
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: 9781603273251
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1603273247
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9781603273244
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Quantification of endogenous retinoids / Maureen A. Kane and Joseph L. Napoli -- Culture of highly differentiated human retinal pigment epithelium for analysis of the poarized uptake, processing, and secretion of retinoids / Jane Hu and Dean Bok -- Feeder-independent culture of mouse embryonic stem cells using vitamin A/retinol / Jaspal S. Khillan and Liguo Chen -- In vitro assays of rod and cone opsin activity : retinoid analogs as agonists and inverse agonists / Masahir Kono and Rosalie K. Crouch -- Physiological studies of the interaction between opsin and chromophore in rod and cone visual pigments / Vladimir J. Kefalov, M. Carter Cornwall, and Gordon L. Fain -- Measurement of the mobility of all-trans-retinol with two-photon fluorescence recovery after photobleaching / Yiannis Koutalos -- Microfluorometric measurement of the formation of all-trans-retinol in the outer segments of single isolated vertebrate photoreceptors / Yiannis Koutalos and M. Carter Cornwall -- HPLC/MSN analysis of retinoids / James E. Evans and Peter McCaffery -- Binding of retinoids to ABCA4, the photoreceptor ABC transporter associated with Stargardt macular degeneration / Ming Zhong and Robert S. Molday -- Fluorescence-based technique for analyzing retinoic acid / Leslie J. Donato and Noa Noy -- The interaction between retinol-binding protein and transthyretin analyzed by florescence anisotropy / Claudia Folli, Roberto Favilla, and Rodolfo Berni -- Assay of retinol-binding protein-transthyretin interaction and techniques to identify competing ligands / Nathan L. Mata, Kim Phan, and Yun Han -- Molecular biology and analytical chemistry methods used to probe the retinoid cycle / Marcin Golczak [and others] -- Visualization of retinoid storage and trafficking by two-photon microscopy / Yoshikazu Imanishi and Krzyztof Palczewski -- Reverse-phase high-performance liquid chromatography (HPLC) analysis of retinol and retinyl esters in mouse serum and tissues / Youn-Kyung Kim and Lredana Quadro -- Detection of retinoic acid catabolism with reporter systems and by in situ hybridization for CYP26 enzymes / Yasuo Sakai and Ursula C. Dräger -- Diet in vitamin A research / A. Catharine Ross -- Experimental approaches to the study of A2E, a bisretinoid lipofuscin chromophore of retinal pigment epithelium, / Janet R. Sparrow, So Ra Kim, and Yalin Wu -- Analysis of the retinoid isomerase activities in the retinal pigment epithelium and retina / Gabriel H. Travis, Joanna Kaylor, and Quan Yuan -- Techniques to study specific cell-surface receptor-mediated cellular vitamin A uptake / Riki Kawaguchi and Hui Sun.
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| Abstract
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New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A's impact on the human body.
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| Subject
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Retinoids.
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| Subject
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Retinoids.
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Retinoids.
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| Dewey Classification
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612.399
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| LC Classification
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QP801.R47
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| NLM classification
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QU 167R442 2010
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| Added Entry
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Sun, Hui,1969-
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Travis, Gabriel H.
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