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" Transcription factors : "
edited by Paul J. Higgins.
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BL
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| Record Number
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965767
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b720137
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| Title & Author
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Transcription factors : : methods and protocols /\ edited by Paul J. Higgins.
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| Publication Statement
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New York :: Humana Press,, ©2010.
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| Series Statement
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Methods in molecular biology,; 647
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Springer protocols
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1 online resource (xiv, 412 pages) :: illustrations (some color)
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| ISBN
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1607617374
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: 1607617382
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: 9781607617372
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: 9781607617389
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1607617374
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9781607617372
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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A review of post-translational modifications and subcellular localization of Ets transcription factors : possible connection with cancer and involvement in the hypoxic response / Céline Charlot [and others] -- Regulation of transcription factor function by targeted protein degradation : an overview focusing on p53, c-Myc, and c-Jun / Jukka Westermarck -- Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents / Aldo Giudice, Claudio Arra, and Maria C. Turco -- Subnuclear localization and intranuclear trafficking of transcription factors / Sayyed K. Zaidi [and others] -- Analysis of ligand-dependent nuclear accumulation of Smads in TGF-[beta] signaling / Douglas A. Chapnick and Xuedong Liu -- Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1 / Richard Y.M. Ma [and others] -- Coupling of dephosphorylation and nuclear export of Smads in TGF-[beta] signaling / Fangyan Dai [and others] -- Assessing sequence-specific DNA binding and transcriptional activity of STAT1 transcription factor / Thomas Meyer and Uwe Vinkemeier -- Analysis of nuclear export using photoactivatable GFP fusion proteins and interspecies heterokaryons / Kerry-Ann Nakrieko, Iordanka A. Ivanova, and Lina Dagnino -- Determination of nuclear localization signal sequences for Krüppel-like factor 8 / Tina S. Mehta, Farah Monzur, and Jihe Zhao -- Methods to measure nuclear export of [beta]-catenin using fixed and live cell assays / Manisha Sharma and Beric R. Henderson -- Imaging of transcription factor trafficking in living cells : lessons from corticosteroid receptor dynamics / Mayumi Nishi -- Hypoxia-inducible factors : post-translational crosstalk of signaling pathways / Elitsa Y. Dimova and Thomas Kietzmann -- The basic helix-loop-helix-leucine zipper gene Mitf : analysis of alternative promoter choice and splicing / Kapil Bharti [and others] -- Phosphorylation control of nuclear receptors / Sébastien Lalevée, Christine Ferry, and Cécile Rochette-Egly -- Regulation of Krüpple-like [i.e. Krüppel-like] factor 5 by targeted protein degradation / Ceshi Chen -- Post-translational control of ETS transcription factors : detection of modified factors at target gene promoters / Li Li, Janice Saxton, and Peter E. Shaw -- Integration of protein kinases into transcription complexes : identifying components of immobilised in vitro pre-initiation complexes / Hong-Mei Zhang [and others] -- Post-translational modification of p53 by ubiquitin / Chunhong Yan -- Phosphorylation-dependent regulation of SATB1, the higher-order chromatin organizer and global gene regulator / Dimple Notani [and others] -- In vivo and in vitro tools to identify and study transcriptional regulation of USF-1 target genes / Marie-Dominique Galibert and Sébastien Corre -- Measuring the absolute abundance of the Smad transcription factors using quantitative immunoblotting / David C. Clarke and Xuedong Liu -- Flow cytometry analysis of transcription factors in T lymphocytes / Diana I. Albu, Danielle Califano, and Dorina Avram -- Identification of specific protein/E-box-containing DNA complexes : lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family / Marie-Dominique Galibert and Yorann Baron.
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| Abstract
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In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Transcription Factors: Methods and Protocols compiles the latest techniques for elucidating controls on transcription factor intracellular localization and activity, and consequently is unlike any other methods-based text on transcriptional regulation today.
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Transcription factors, Laboratory manuals.
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Transcription factors-- Research-- Methodology.
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Animal Biochemistry.
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Health Biological Sciences.
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Human Anatomy Physiology.
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Transcription factors.
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Transcription Factors.
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| Dewey Classification
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572.8845
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| LC Classification
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QP552.T68T745 2010
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| NLM classification
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QU 475T767 2010
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| Added Entry
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Higgins, Paul J.
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