| Document Type
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BL
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| Record Number
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968421
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b722791
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| Main Entry
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Author, Unknown.
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| Title & Author
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METHODS IN CELL BIOLOGY, VOLUME 28 : : DICTYOSTELIUM DISCOIDEUM: MOLECULAR APPROACHES TO CELL BIOLOGY.
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| Publication Statement
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Burlington :: Elsevier Science,, 1987.
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| Series Statement
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Methods in Cell Biology ;; v. 28
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| Page. NO
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1 online resource (533 pages).
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| ISBN
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0080859267
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: 9780080859262
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| Notes
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Chapter 10. Ultrastructural Localization of Cytoskeletal Proteins in Dictyostelium Amoebae.
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| Contents
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Front Cover -- Dictyostelium discoideum: Molecular Approaches to Cell Biology -- Copyright Page -- CONTENTS -- Preface -- PART I: GENERAL PRINCIPLES -- Chapter 1. Introductory Remarks and Some Biochemical Considerations -- I. Introductory Remarks -- ll. Some Biochemical Considerations -- lll. Concluding Remarks -- References -- Chapter 2. Cultivation and Synchronous Morphogenesis of Dictyostelium under Controlled Experimental Conditions -- l. Introduction -- ll. Cell Cultivation -- lll. Strain Maintenance -- IV. Strain Preservation -- V. Conditions for Synchronous Development.
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I. Introduction -- ll. Quantitation of Endocytotic Uptake -- lll. Adhesion Assay -- IV. Aggregation Assay -- V. Isolation of Mutants with Altered Cohesive Properties -- VI. Characterization of Mutants -- VII. Conclusions and Discussion -- References -- Chapter 7. Defining the Intracellular Localization Pathways followed by Lysosomal Enzymes in Dictyostelium discoideum -- I. Introduction -- ll. Pulse-Chase Labeling Analyses of Lysosomal Enzymes -- lll. Subcellular Fractionation -- IV. Synthesis in Vitro of Lysosomal Enzymes.
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IV. Mapping and Sequencing of Dictyostelium Genes -- V. Analysis of Transcripts -- VI. DNA-Mediated Transformation -- VII. Appendix -- References -- PART ll: VESICULAR TRAFFIC IN CELLS -- Chapter 5 . Purification and Characterization of Dictyostelium discoideum Plasma Membranes -- I. Introduction -- ll. Membrane Purification Procedures -- lll. Comparison of Plasma Membranes Obtained by Different Methods -- IV. Vesiculation and Sealing of Plasma Membranes -- V. Choosing a Membrane Isolation Procedure -- References -- Chapter 6 . Endocytosis and Recognition Mechanisms in Dictyostelium discoideum.
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V. Isolation and Characterization of Mutants Altered in the Modification, Processing, and Secretion of Lysosomal Enzymes -- VI. Summary and Future Perspectives -- References -- Chapter 8. The Axenic Mutations and Endocytosis in Dictyostelium -- l. Introduction -- ll. Methods -- lll. Discussion -- References -- PART lll: CELL MOTILITY -- Chapter 9. Introduction of Exogenous Molecules into the Cytoplasm of Dictyostelium discoideum Amoebae by Controlled Sonication -- I. Introduction -- ll. Sonication Loading -- lll. Applications of Sonication Loading -- References.
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VI. Appendix: Some Useful Media and Solutions -- References -- Chapter 3. Genetic Tools for Dictyostelium discoideum -- l. Introduction -- ll. Mutagenesis -- lll. Selections -- IV. Screening Techniques -- V. Mutant Analysis -- Vl. Genetic Exchange -- VII. Mitotic Crossing Over -- VIII. Mapping Cloned Sequences -- IX. DNA Transformation -- X . Discussion -- XI. Sample Genetic Study -- References -- Chapter 4. Molecular Biology in Dictyostelium: Tools and Applications -- I. Introduction -- ll. Nucleic Acid Preparations -- lll. Recombinant DNA Libraries.
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| Abstract
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METHODS IN CELL BIOLOGY, VOLUME 28: DICTYOSTELIUM DISCOIDEUM: MOLECULAR APPROACHES TO CELL BIOLOGY.
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| Subject
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Cytology.
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| Subject
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Dictyostelium discoideum.
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| Dewey Classification
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591.59
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| LC Classification
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QH585 -- M4828 1987eb
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| Added Entry
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Unknown
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