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" Bioengineering and molecular biology of plant pathways / "
edited by Hans Bohnert, Henry Nguyen, Norman G. Lewis.
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BL
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| Record Number
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969129
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b723499
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| Title & Author
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Bioengineering and molecular biology of plant pathways /\ edited by Hans Bohnert, Henry Nguyen, Norman G. Lewis.
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| Publication Statement
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Amsterdam ;Boston :: Pergamon,, 2008.
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| Series Statement
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Advances in plant biochemistry and molecular biology ;; v. 1
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1 online resource (xxiii, 455, 28 pages) :: illustrations.
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| ISBN
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0080449727
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: 0080556965
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: 1281144703
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: 6611144706
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: 9780080449722
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: 9780080556963
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: 9781281144706
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: 9786611144708
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0080449727
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9780080449722
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Front Cover; Bioengineering and Molecular Biology of Plant Pathways; Copyright Page; Dedication Page; Contents; Contributors; Introduction to the Series and Acknowledgements; Preface to Volume 1; Prologue; Chapter 1: Metabolic Organization in Plants: A Challenge for the Metabolic Engineer; 1. Introduction; 2. Plant Metabolic Networks and Their Organization; 3. Tools for Analyzing Network Structure and Performance; 3.1. Constraints-based network analysis; 3.2. Metabolic flux analysis; 3.3. Kinetic modeling; 3.4. Metabolic control analysis; 4. Integration of Plant Metabolism
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2.2. Genomic analysis suggests most enzymes evolve from preexisting enzymes2.3. Evolution of a new enzymatic activity in nature; 2.4. The natural evolution process initially produces poor enzymes; 2.5. Sequence space and fitness landscapes; 3. Practical Considerations for Engineering Enzymes; 3.1. Identifying appropriate starting enzyme(s); 3.2. Ways of introducing variability into genes; 3.3. Choice of expression system; 3.4. Identifying improved variants; 3.5. Recombination and/or introduction of subsequent mutations; 3.6. Structure-based rational design
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2.3. Glutamate dehydrogenase: An enzyme with controversial functions in plants2.4. The network of amide amino acids metabolism is regulated in concert by developmental, physiological, environmental, metabolic, and stress-derived signals; 3. The Aspartate Family Pathway that is Responsible for Synthesis of the Essential Amino Acids Lysine, Threonine, Methionine, and Isoleucine; 3.1. The aspartate family pathway is regulated by several feedback inhibition loops; 3.2. Metabolic fluxes of the aspartate family pathway are regulated by developmental, physiological, and environmental signals
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4. Opportunities for Plant Improvement Through Engineered Enzymes and Proteins4.1. Challenges for engineering plant enzymes and pathways; 5. Summary; Acknowledgements; References; Chapter 3: Genetic Engineering of Amino Acid Metabolism in Plants; 1. Introduction; 2. Glutamine, Glutamate, Aspartate, and Asparagine are Central Regulators of Nitrogen Assimilation, Metabolism, and Transport; 2.1. GS: A highly regulated, multifunctional gene family; 2.2. Role of the ferredoxin- and NADH-dependent GOGAT isozymes in plant glutamate biosynthesis
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4.1. Relationship between enzyme properties and network fluxes4.2. Limitations on metabolic compensation within a network; 4.3. Impact of physiological conditions on network performance; 4.4. Network adjustments through alternative pathways; 4.5. Propagation of metabolic perturbations through networks; 4.6. Enzyme-specific responses within networks; 4.7. Impact of metabolic change on network structure; 5. Summary; Acknowledgements; References; Chapter 2: Enzyme Engineering; 1. Introduction; 2. Theoretical Considerations; 2.1. Enzyme architecture is conserved
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| Abstract
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The increased knowledge about the structure of genomes in a number of species, about the complexity of transcriptomes, and the rapid growth in knowledge about mutant phenotypes have set off the large scale use of transgenes to answer basic biological questions, and to generate new crops and novel products. This volume includes twelve chapters, which to variable degrees describe the use of transgenic plants to explore possibilities and approaches for the modification of plant metabolism, adaptation or development. The interests of the authors range from tool development, to basic biochemical know-how about the engineering of enzymes, to exploring avenues for the modification of complex multigenic pathways, and include several examples for the engineering of specific pathways in different organs and developmental stages. * Prologue by Paul K. Stumpf and Eric E. Conn * Incorporates new concepts and insights in plant biochemistry and biology * Provides a conceptual framework regarding the challenges faced in engineering pathways * Discusses potential in engineering of metabolic end-products that are of vast economical importance including genetic engineering of cellulose, seed storage proteins, and edible and industrial oils.
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Botanical chemistry.
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Plant biotechnology.
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Plant molecular biology.
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Botanical chemistry.
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Plant biotechnology.
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Plant molecular biology.
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SCIENCE-- Life Sciences-- Biochemistry.
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| Dewey Classification
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572.2
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| LC Classification
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TP248.27.P55B56 2008eb
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| Added Entry
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Bohnert, Hans J.
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Lewis, Norman G.,1949-
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Nguyen, Henry T.
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