| Document Type
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BL
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| Record Number
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970380
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| Doc. No
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b724750
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| Title & Author
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Growth factors and receptors : : a practical approach /\ edited by Ian A. McKay and Kenneth D. Brown.
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| Publication Statement
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Oxford [England] ;New York :: Oxford University Press,, 1998.
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| Series Statement
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Practical approach series ;; 194
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| Page. NO
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1 online resource (xxii, 266 pages) :: illustrations.
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| ISBN
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0191565652
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: 0585483973
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: 9780191565656
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: 9780585483979
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Intro; Contents; List of contributors; Abbreviations; 1. Engineered growth factors and receptors, and their applications; 1. Introduction; Known growth factors and receptors; Modes of factor-receptor interaction; 2. Applications of engineered growth factors and receptors; 3. Principal methods for engineering growth factors and their receptors; Mutagenesis; Formation of chimeric proteins; Manufacture of alternative ligands and receptors; 4. Design of engineered growth factors and receptors; DNA sequence of factor or receptor to be engineered.
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Analysis of existing DNA sequence for useful restriction sitesDesign of wholly synthetic growth factors and receptors; 5. Expression of DNAs encoding growth factors or receptors; Deciding on an expression system; Microbial expression; In vitro expression; Eukaryotic gene expression; Acknowledgements; References; 2. Engineering of novel neurotrophins; 1. Introduction; 2. Structure-function analyses of neurotrophins; 3. Site-directed mutagenesis; Kunkel method; PCR-based mutagenesis; 4. Expression of recombinant protein; COS cell expression; Baculovirus expression.
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| Abstract
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Growth factors are extra-cellular molecules that interact with receptors on the cell surface to initiate a sequence of events culminating in transcription of DNA prior to cell division. Disturbances in the production of growth factors or the sensitivity of their receptors are important in tumour growth. Therefore, there is much interest in engineering both growth factors and their receptors to intervene in cancer. This book describes a variety of techniques for theengineering, analysis, definition of function and detection of gene expression of growth factors and their receptors. A number of p.
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| Subject
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Gene therapy-- Research, Laboratory manuals.
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| Subject
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Genetic engineering, Laboratory manuals.
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| Subject
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Growth factors, Laboratory manuals.
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| Subject
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Growth factors-- Receptors, Laboratory manuals.
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| Subject
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Gene therapy-- Research.
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| Subject
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Genetic engineering.
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| Subject
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Growth factors-- Receptors.
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| Subject
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Growth factors.
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| Subject
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SCIENCE-- Life Sciences-- Developmental Biology.
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| Subject
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Growth Substances, Laboratory Manuals.
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| Subject
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Genetic Therapy, Laboratory Manuals.
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| Subject
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Receptors, Growth Factor, Laboratory Manuals.
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| Dewey Classification
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571.8/4
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| LC Classification
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QP552.G76G7445 1998eb
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| NLM classification
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1998 G-931
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QU 25G884 1998
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| Added Entry
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Brown, Kenneth D.
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McKay, Ian, (Ian A.)
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