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" Capillary electrophoresis of nucleic acids. "
edited by Keith R. Mitchelson and Jing Cheng.
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BL
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| Record Number
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972722
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b727092
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| Title & Author
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Capillary electrophoresis of nucleic acids.\ edited by Keith R. Mitchelson and Jing Cheng.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©2001.
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| Series Statement
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Methods in molecular biology ;; v. 163, pt. 2
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| Page. NO
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1 online resource (1 volume) :: illustrations
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| ISBN
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1592591167
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: 9781592591169
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Development of a high-throughput capillary electrophoresis protocol for DNA fragment analysis / H.M. Wenz, D. Dailey and M.D. Johnson -- Ultra-fast DNA separations using capillary electrophoresis / K. Kleparnik, O.M. Mueller and F. Foret -- Fast DNA fragment sizing in a short capillary column / H.J. Issaq and K.C. Chan -- Detection of DNA polymorphisms using pcr-rflp and capillary electrophoresis / J.M. Butler and D.J. Reeder -- High resolution analysis of point mutations by constant denaturant capillary electrophoresis (cdce) / K. Khrapko [and others] -- Point mutation detection by temperature-programmed capillary electrophoresis / C. Gelfi [and others] -- Single-nucleotide primer extension assay by capillary electrophoresis laser-induced fluorescence / C.A. Piggee and B.L. Karger -- Amplification refractory mutation system analysis of point mutations by capillary electrophoresis / P. Carrera [and others] -- Sscp analysis of point mutations by multicolor capillary electrophoresis / K. Hayashi [and others] -- Sscp analysis by capillary electrophoresis with laser-induced fluorescence detector / J. Ren -- Magnetic bead-isolated single-strand DNA for sscp analysis / T. Kasuga, J. Cheng and K.R. Mitchelson -- Analysis of short tandem repeat markers by capillary array electrophoresis / E.S. Mansfield, R.B. Wilson and P. Fortina -- Genotyping by microdevice electrophoresis / D. Schmalzing [and others] -- Applications of constant denaturant capillary electrophoresis and complementary procedures. Measurement of point mutational spectra / A.S. Kim, X.C. Li-Sucholeiki and W.G. Thilly -- Toward effective pcr-based amplification of DNA on microfabricated chips / J.P. Ferrance, B. Giordano and J.P. Landers -- Low-stringency single specific primer-amplified DNA analysis by capillary electrophoresis / M.A. Marino -- Dop-pcr amplification of whole genomic DNA and microchip-based capillary electrophoresis / P. Fortina [and others] -- Analysis of triplet-repeat DNA by capillary electrophoresis / Y. Kiba and Y. Baba -- Chemical mismatch cleavage analysis by capillary electrophoresis with laser-induced fluorescence detection / J. Ren -- Quantitation of mrna by competitive pcr using capillary electrophoresis / S.J. Williams and P.M. Williams -- Quantitative rt-pcr from fixed paraffin-embedded tissues by capillary electrophoresis / G. Stanta, S. Bonin and M. Lugli -- Differential display analysis by capillary electrophoresis / X. Zhao and K.S. George -- Capillary array electrophoresis analyzer / M. Kamahori and H. Kambara -- DNA sequencing at elevated temperature by capillary electrophoresis / P. Lindberg and J. Roeraade -- DNA sequencing in noncovalently coated capillaries using low viscosity polymer solutions / R. Madabhushi -- Selective primer sequencing from a DNA mixture by capillary electrophoresis / T. Li, K. Okano and H. Kambara -- Sequencing of rapd fragments using 3'-extended oligonucleotide primers / J. Davis [and others] -- Capillary affinity gel electrophoresis / Y. Baba -- Capillary DNA-protein mobility shift assay / J. Xian -- Protein-DNA binding affinities by capillary electrophoresis / G.J. Foulds and F.A. Etzkorn -- Ligand binding to oligonucleotides / Hamdan, II, G.G. Skellern and R.D. Waigh.
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| Abstract
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The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has produced a host of DNA analytical procedures. In the Unprecedentedly wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This second volume of the set, Practical Applications of Capillary Electrophoresis, covers techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions. Several chapters also discuss CE analysis at elevated temperatures and the use of micro-CE and array-CE devices. The companion volume, Introduction to the Capillary Electrophoresis of Nucleic Acids, offers readily reproducible methods for the analysis of small oligonucleotides and modified nucleotides, and time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE. Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today.
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| Subject
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Capillary electrophoresis.
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Nucleic acids-- Separation.
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Capillary electrophoresis.
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Nucleic acids-- Separation.
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SCIENCE-- Life Sciences-- Molecular Biology.
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| Dewey Classification
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572.8
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| LC Classification
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QP620.C36eb vol. 2
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| Added Entry
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Cheng, Jing,1963-
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Mitchelson, Keith R.
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| Parallel Title
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Practical applications of capillary electrophoresis
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