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BL
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| Record Number
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974050
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| Doc. No
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b728420
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| Title & Author
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Emerging technologies in protein and genomic material anaylsis\ edited by György Marko-Varga and Peter L. Oroszlan.
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| Publication Statement
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Amsterdam ;Boston :: Elsevier,, 2003.
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| Series Statement
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Journal of chromatography library,; v. 68
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| Page. NO
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1 online resource (295 p.).
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| ISBN
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0080530842
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: 1281055018
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: 6611055010
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: 9780080530840
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: 9781281055019
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: 9786611055011
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| Notes
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7.3. Application to the analysis of IL-8 and IL-10 in cell samples
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Description based upon print version of record.
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Front Cover; Emerging Technologies in Protein and Genomic Material Analysis; Copyright Page; Contents; Preface; List of Contributors; Chapter 1. Enabling Bio-analytical Technologies for Protein and Genomic Material Analysis and their Impact on Biology; 1.1. Background; 1.2. Industrial impact; 1.3. Proteomics -- the new frontier after the human genome; 1.4. Protein chip arrays; 1.5. DNA/RNA analysis; 1.6. Miniaturized, micro-scale systems; 1.7. Concluding remarks; 1.8. References; Chapter 2. DNA Sequencing: From Capillaries to Microchips; 2.1. Introduction
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2.2. The early days of automated DNA sequencing2.3. The advent of capillary gel electrophoresis; 2.4. Towards higher throughput: capillary array electrophoresis; 2.5. The promise of miniaturization: microchips; 2.6. Dealing with the data: bioinformatics; 2.7. Conclusions; 2.8. References; Chapter 3. Phosphoprotein and Phosphoproteome Analysis by Mass Spectrometry; 3.1. Introduction; 3.2. Phosphoprotein analysis: A challenge for mass spectrometry; 3.3. Mass spectrometry based phosphatase/kinase assays; 3.4. Phosphoprotein and phosphopeptide enrichment strategies
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3.5. Chemical derivatization of phosphopeptides and phosphoproteins3.6. Selective detection and sequencing of phosphopeptides by tandem mass spectrometry; 3.7. Liquid chromatography-tandem mass spectrometry (LC-MS/MS); 3.8. Phosphoproteome analysis by mass spectrometry; 3.9. Emerging MS methods tot phosphoprotein analysis; 3.10. Relative quantitation of phosphorylation site occupancy by stable isotope labeling of proteins; 3.11. Conclusion; 3.12. Acknowledgements; 3.13. References
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5.6. References5.7. Appendix; Chapter 6. Capillary Isoelectric Focusing Developments in Protein Analysis; 6.1. Introduction; 6.2. Detection; 6.3. Micropreparative CIEF; 6.4. Internal standards; 6.5. Capillary coatings; 6.6. CIEF applications; 6.7. CIEF-mass spectrometry; 6.8. Miniaturized CIEF and chip-IEF; 6.9. References; Chapter 7. Bio-affinity Extraction for the Analysis of Cytokines and Proteomics Samples; 7.1. Introduction to cytokine analysis; 7.2. Capillary micro extraction linked to MALDI-TOF MS for the analysis of cytokines at femtomole levels
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Chapter 4. Quantitative Peptide Determination Using Column-Switching Capillary Chromatography Interfaced with Mass Spectrometry4.1. Introduction; 4.2. Instrumentation; 4.3. Examples of quantitative peptide analysis; 4.4. Resume; 4.5. Acknowledgements; 4.6. References; Chapter 5. On-line Continuous-flow Multi-protein Biochemical Assays for the Characterization of Bio-active Compounds Using Electrospray Quadrupole Time-of-Flight Mass Spectrometry; 5.1. Introduction; 5.2. Experimental; 5.3. Results and discussion; 5.4. On-line continuous-flow MS-based biochemical interaction; 5.5. Conclusions
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| Abstract
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It is widely recognized that analytical technologies and techniques are playing a pioneering role in a range of today's foremost challenging scientific endeavours, including especially biological and biomedical research. Worthy of mention, for example, are the role that high performance separation techniques played in mapping the human genome and the pioneering work done within mass spectrometry. It is also apparent that state-of-the-art pharmaceutical and biomedical research is the major driving force of the development of new analytical techniques. Advancements in genomics research h.
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| Subject
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Chromatographic analysis.
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Mass spectrometry.
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Nucleotide sequence.
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Proteins-- Analysis.
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Chromatographic analysis.
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Mass spectrometry.
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Nucleotide sequence.
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Proteins-- Analysis.
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| Dewey Classification
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572.6572/.6 21
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| LC Classification
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QP551.E53 2003
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| Added Entry
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Marko-Varga, György A.
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Oroszlan, Peter L.
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