Document Type
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BL
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Record Number
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975381
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Doc. No
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b729751
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Main Entry
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Author, Unknown.
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Title & Author
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METHODS IN CELL BIOLOGY, VOLUME 31 : : VESICULAR TRANSPORT, PART A.
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Publication Statement
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Burlington :: Elsevier Science,, 1989.
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Series Statement
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Methods in Cell Biology ;; v. 31
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Page. NO
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1 online resource (627 pages).
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ISBN
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0080859291
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: 9780080859293
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Notes
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Chapter 9. Delivery of Macromolecules into Cells Expressing a Viral Membrane Fusion Protein.
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Contents
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Front Cover -- Methods in Cell Biology, Volume 31 -- Copyright Page -- Contents -- Contributors -- Preface -- PART I: GAINING ACCESS TO THE CYTOPLASM -- Chapter 1. Lysed Chromatophores: A Model System for the Study of Bidirectional Organelle Transport -- I. Introduction -- II. Methods to Obtain, Lyse, and Reactivate Chromatophores -- III. Mechanisms of Organelle Transport in Chromatophores -- IV. Summary -- References -- Chapter 2. Digitonin Permeabilization Procedures for the Study of Endosome Acidification and Function -- I. Introduction.
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II. Preparation of Transport-Competent Membranes -- III. Reconstitution of Protein Transport -- IV. Future Prospects -- References -- Chapter 8. Reconstitution of Transport from the ER to the Golgi Complex in Yeast Using Microsomes and Permeabilized Yeast Cells -- I. Introduction -- II. In Vitro Translation of Prepro-<U+00dc>-factor in a Yeast Lysate -- III. In Vitro Translocation into the ER Lumen of PYC or Microsomes -- IV. In Vitro Transport of Pro-<U+00dc>-factor from the ER to the Golgi Complex -- V. Summary -- References.
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II. Prepartion of Mannose-BSA for Endosome Acidification Studies -- III. Permeabilization of Cells -- IV. Endosome Acidification -- V. Summary and Outlook -- References -- Chapter 3. ATP Permeabilization of the Plasma Membrane -- I. Introduction -- II. Plasma Membrane Receptors for Adenine Nucleotides -- III. ATP Permeabilization -- IV. Recovery of Intracellular Homeostasis following ATP Permeabilization -- V. Assessment of Permeabilization -- VI. ATP-Resistant Cells -- VII. Uses of ATP Permeabilization -- VIII. Relative Merits of ATP as a Means of Membrane Permeabilization.
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III. Preparation of Semiintact Cells from Adherent Cells -- IV. Preparation of Semiintact Cells from Suspension Cells -- V. Incubation Conditions to Achieve Transport between the ER and Golgi in Vitro -- VI. Discussion -- References -- Chapter 6. Perforated Cells for Studying Intracellular Membrane transport -- I. Introduction -- II. Generation and Characterization of Perforated Cells -- III. Reconstitution of Intracellular Membrane transport -- IV. Discussion -- References -- Chapter 7. Reconstitution of Protein Transport Using Broken Yeast Spheroplasts -- I. Introduction.
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IX. Mechanism of ATP-Induced Permeabilization -- X. Conclusion -- References -- Chapter 4. Poration by <U+00dc>-Toxin and Streptolysin 0: An Approach to Analyze Intracellular Processes -- I. Introduction -- II. Purification and Analysis of Pore-Forming Toxins -- III. Application for Cell Poration -- IV. Pore-Forming Toxins as Tools in the Study of Intracellular Processes -- V. Concluding Remarks -- References -- Chapter 5. Preparation of Semiintact Chinese Hamster Ovary Cells for Reconstitution of Endoplasmic Reticulum-to-Golgi transport in a Cell-Free System -- I. Introduction -- II. Growth of Cells.
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Abstract
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METHODS IN CELL BIOLOGY, VOLUME 31: VESICULAR TRANSPORT, PART A.
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Subject
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Biological transport.
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Subject
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Endocytosis.
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Dewey Classification
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571.6
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LC Classification
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QH634 -- M4831 1989eb
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Added Entry
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Unknown
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