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" Cell-based assays for high-throughput screening : "
edited by Paul A. Clemons, Nicola J. Tolliday, and Bridget K. Wagner.
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BL
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| Record Number
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976263
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b730633
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| Title & Author
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Cell-based assays for high-throughput screening : : methods and protocols /\ edited by Paul A. Clemons, Nicola J. Tolliday, and Bridget K. Wagner.
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| Publication Statement
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New York :: Humana,, ©2009.
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| Series Statement
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Methods in molecular biology,; v. 486
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Springer protocols
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1 online resource (x, 218 pages) :: illustrations
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| ISBN
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1603275444
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: 1603275452
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: 9781603275446
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: 9781603275453
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Introduction: Cell-based assays for high-throughput screening / W.F. An and N.J. Tolliday -- High-throughput screening of model bacteria / S. Zlitni, J.E. Blanchard and E.D. Brown -- Cell-based assays to probe the ERK MAP kinase pathway in endothelial cells / M.R. Wyler [and others] -- Large-scale small-molecule screen using zebrafish embryos / C.C. Hong -- Whole-animal high-throughput screens: The C. elegans model / E.J. O'Rourke, A.L. Conery and T.I. Moy -- Whole-organism screening: Plants / A. Agee and D. Carter -- Fluorescence-based assays / W.F. An -- Reporter gene assays / A.M.F. Liu [and others] -- Screening for chemical inhibitors of heterologous proteins expressed in yeast using a simple growth-restoration assay / A.D. Balgi and M. Roberge -- Assay for isolation of inhibitors of her2-kinase expression / G. Chiosis and A.B. Keeton -- High-content screening: Flow cytometry analysis / B.S. Edwards [and others] -- High-throughput real-time PCR for detection of gene-expression levels / B.K. Wagner and Z. Arany -- Interpretation of uniform-well readouts / S. Josiah -- Extracting rich information from images / A.E. Carpenter.
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| Abstract
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As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
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Biological assay.
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High throughput screening (Drug development)
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Biochemistry.
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Biological assay.
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Biology - General.
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Biology.
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Chemistry.
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Essais biologiques.
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Health Biological Sciences.
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High throughput screening (Drug development)
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High throughput screening
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Pharmacie-- Recherche.
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Pharmacy, Therapeutics, Pharmacology.
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Physical Sciences Mathematics.
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Untersuchungsmethode
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Zelle.
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Drug Discovery.
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Biological Assay.
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Drug Evaluation, Preclinical.
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Dosage biologique-- Cahiers de laboratoire.
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Évaluation préclinique médicament-- Cahiers de laboratoire.
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| Dewey Classification
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615/.19076
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| LC Classification
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RS419.5.C45 2009
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| NLM classification
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QV 25C393 2009
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W1ME9616J v.486 2009
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| Added Entry
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Clemons, Paul A.
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Tolliday, Nicola J.
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Wagner, Bridget K.
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