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" Human cell culture protocols. "
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BL
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| Record Number
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979336
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b733706
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| Title & Author
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Human cell culture protocols.
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| Edition Statement
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2nd ed. // edited by Joanna Picot.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©2005.
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| Series Statement
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Methods in molecular medicine ;; 107
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| Page. NO
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1 online resource (xi, 359 pages) :: illustrations
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| ISBN
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1280359544
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: 1588292223
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: 1592598617
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: 6610359547
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: 9781280359545
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: 9781588292223
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: 9781592598618
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: 9786610359547
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Establishment and maintenance of normal human keratinocyte cultures / Claire Linge -- Cultivation of normal human epidermal melanocytes in the absence of phorbol esters / Mei-Yu Hsu, Ling Li, and Meenhard Herlyn -- Isolation and culture of human osteoblasts / Alison Gartland [and others] -- Human osteoclast culture from peripheral blood monocytes : phenotypic characterization and quantitation of resorption / Katherine A. Buckley [and others] -- Human chondrocyte cultures as models of cartilage-specific gene regulation / Mary B. Goldring -- Human myoblasts and muscle-derived SP cells / Grace K. Pavlath and Emanuela Gussons -- Cell cultures of autopsy-derived fibroblasts / Volker Meske, Frank Albert, and Thomas G. Ohm -- Culture and differentiation of human adipocyte precursor cells in primary culture / Vanessa van Harmelen, Thomas Skurk, and Hans Hauner -- Human mononuclear phagocytes in tissue culture / Yona Keisari -- Purification of peripheral blood natural killer cells / Bice Perussia and Matthew J. Loza -- Human fetal brain cell culture / Mark P. Mattson -- Culturing human schwann cells / Victor J. Turnbull.
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Well-differentiated human airway epithelial cell cultures / M. Leslie Fulcher [and others] -- Isolation and culture of human alveolar epithelial cells / Carsten Ehrhardt, Kwang-Jin Kim, and Claus-Michael Lehr -- A new approach to primary culture of human gastric epithelium / Pierre Chailler and Daniel MÄnard -- Isolation and culture of human colon epithelial cells using a modified explant technique employing a noninjurious approach / Hamid A. Mohammadpour -- Isolation and culture of human hepatocytes / Martin Bayliss and Graham Somers -- Glomerular epithelial and mesangial cell culture and characterization / Heather M. Wilson and Keith N. Stewart -- Isolation and culture of human renal cortical cells with characteristics of proximal tubules / Gabrielle M. Hawksworth -- Culture of parathyroid cells / Per Hellman -- Long-term culture and maintenance of human islets of langerhans in memphis serum-free media / Daniel W. Fraga -- Primary culture of human antral endocrine and epithelial cells / Susan B. Curtis and Alison M.J. Buchan -- Conjunctiva organ and cell culture / Monica Berry and Marcus Radburn-Smith -- Establishment and maintenance of in vitro cultures of human retinal pigment epithelium / Martin J. Stevens.
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| Abstract
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In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. Each technique can be used to investigate a wide spectrum of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to control of proliferation and differentiation. New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today.
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| Subject
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Human cell culture, Laboratory manuals.
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Cellules humaines-- Culture, Manuels de laboratoire.
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Human cell culture.
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MEDICAL-- Research.
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Cells, Cultured.
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Cytological Techniques.
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Celkweek.
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Protocollen (zorgsector)
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| Subject
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Wetenschappelijke technieken.
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| Dewey Classification
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616/.0277
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| LC Classification
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QH585.2.H85 2005
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| NLM classification
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QS 525H9175 2005
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| Added Entry
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Picot, Joanna.
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