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" Drug-DNA interaction protocols / "
edited by Keith R. Fox.
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BL
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| Record Number
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980700
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b735070
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| Title & Author
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Drug-DNA interaction protocols /\ edited by Keith R. Fox.
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| Edition Statement
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2nd ed.
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| Publication Statement
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New York, N.Y. :: Humana Press,, ©2010.
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| Series Statement
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Methods in molecular biology,; v. 613
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Springer protocols
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| Page. NO
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1 online resource (xi, 318 pages) :: illustrations (some color)
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| ISBN
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1603274189
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: 9781603274180
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1603274170
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9781603274173
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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1. Quantitative analysis of small molecule-nucleic acid interactions with a biosensor surface and surface plasmon resonance detection / Yang Liu and W. David Wilson -- 2. Thermal melting studies of ligand DNA interactions / Aurore Guédin, Laurent Lacroix, and Jean-Louis Mergny -- 3. Circular and linear dichroism of drug-DNA systems / Alison Rodger -- 4. Drug binding to DNA-RNA hybrid structures / Richard T. Wheelhouse and Jonathan B. Chaires -- 5. Quantification of binding data using capillary electrophoresis / Fitsumbirhan Araya, Graham G. Skellern, and Roger D. Waigh -- 6. Determination of equilibrium association constants of ligand-DNA complexes by electrospray mass spectrometry / Valérie Gabelica -- 7. Detection of adriamycin-DNA adducts by accelerator mass spectrometry / Kate Coldwell [and others] -- 8. Molecular modelling methods to quantitate drug-DNA interactions / Hao Wang and Charles A. Laughton -- 9. Application of anomalous diffraction methods to the study of DNA and DNA-complexes / Derrick Watkins [and others] -- 10. DNase I footprinting / Antonia S. Cardew and Keith R. Fox -- 11. Methods to characterize the effect of DNA-modifying compounds on nucleosomal DNA / Vidya Subramanian [and others] -- 12. REPSA: combinatorial approach for identifying preferred drug-DNA binding sequences / Michael W. Van Dyke -- 13. In vitro transcription assay for resolution of drug-DNA interactions at defined DNA sequences / Benny J. Evison, Don. R. Phillips, and Suzanne M. Cutts -- 14. In virtro footprinting of promoter regions within supercoiled plasmid DNA / Daekyu Sun -- 15. Topoisomerase I-mediated DNA relaxation as a tool to study intercalation of small molecules and supercoiled DNA / Paul Peixoto [and others] -- 16. A high-throughput assay for DNA topoisomerases and other enzymes based on DNA triplex formation / Matthew R. Burrell, Nicolas P. Burton and Anthony Maxwell -- 17. Measurement of DNA interstrand crosslinking in individual cells using the single cell gel electrophoresis (Comet) assay / Victoria J. Spanswick, Janet M. Hartley, and John A. Hartley -- 18. Measurement of DNA interstrand crosslinking in naked DNA using Gel-based methods / Konstantinous Kiakos, Janet M. Hartley, and John A. Hartley -- 19. An evaluation cascade for G-quadruplex telomere targeting agents in human cancer cells / Mekala Gunaratnam and Stephen Neidle.
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| Abstract
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The last few years have witnessed the creation of new generations of sequence reading compounds, which have incredible potential for targeting specific DNA sequences. In Drug-DNA Interaction Protocols, Second Edition, expert researchers explore these compounds, revealing how a deeper understanding of the structure of DNA, along with an improved ability to manipulate it, has led to much progress in recent years. Chapters describe a new arsenal of techniques for probing the interactions between drugs and DNA, including various biophysical techniques for quantifying these interactions and for describing them in molecular and atomic detail, and they comprehensively consider both molecular and cellular approaches. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and current, Drug-DNA Interaction Protocols, Second Edition, is a critical volume that includes a wide range of chapters, which together provide methods for assessing the strength and mode of binding, the sequence selectivity, and the effect on biological systems.
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DNA-drug interactions.
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DNA Footprinting-- methods.
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DNA Probes.
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DNA-- drug effects.
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DNA-Binding Proteins.
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Polymerase Chain Reaction-- methods.
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DNA-drug interactions.
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| Dewey Classification
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615/.7045
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| LC Classification
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QP624.75.D77
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| NLM classification
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QU 58.5
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| Added Entry
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Fox, Keith R.
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