| Document Type
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BL
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| Record Number
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990844
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| Doc. No
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b745214
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| Title & Author
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Bacterial toxins : : methods and protocols /\ edited by Otto Holst.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©2000.
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| Series Statement
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Methods in molecular biology ;; 145
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| Page. NO
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xi, 379 pages :: illustrations ;; 24 cm
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| ISBN
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0896036049
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: 9780896036048
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Bacterial protein toxins: an overview -- Purification of clostridium botulinum type A neurotoxin -- Shiga toxins -- Isolation and detection of microcystins and nodularins, cyanobacterial peptide hepatotoxins -- Genetic construction, expression, and characterization of diphtheria toxin-based growth factor fusion proteins -- Structure-function analysis of cysteine-engineered entomopathogenic toxins -- Use of fourier-transformed infrared spectroscopy for secondary structrue determination of staphyloccoccal pore-forming toxins -- The use of fluorescence resonance energy transfer to detect conformational changes in protein toxins -- Site-directed spin labeling of proteins: applications to diphtheria toxin -- Characterization of molecular properties of pore-forming toxins with planar lipid bilayers --Determination of affinity and kinetic rate constants using surface plasmon resonance -- ADP-ribosylation of [alpha]-Gi proteins by pertusis toxin: positional dissection of acceptor sites using membrane anchored synthetic peptides -- Phage libraries for generation of anti-botulinum scFv antibiodies -- T-cell cytotoxicity assays for studying the functional itneraction between the superantigen staphylococcal enterotoxin A and T-cell receptors -- In vitro physiological studies on clostridial neurotoxins: biological models and procedures for extracellular and intracellular application of toxins -- The biology of endotoxin -- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of lipopolysaccharides -- Applications of combined capillary electrophoresis-electrospray mass spectrometry in the characterization of short-chain lipopolysaccharides: haemophilus influenzae -- Deacylation of lipopolysaccharides and isolation of oligosaccharide phosphates -- Electrophysiological measurement on reconstituted outer membranes.
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| Subject
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Bacterial toxins-- Research-- Methodology.
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Bacterial toxins-- Research-- Methodology.
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Bakteriengift
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Biochemische Analyse
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Methode
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Toxikologie
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Toxikologische Analyse
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Toxines bactériennes-- Recherche-- Méthodologie.
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Toxines bactériennes.
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Bakteriengift.
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Biochemische Analyse.
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Methode.
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Toxikologie.
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Toxikologische Analyse.
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Bacterial Toxins.
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Bacteriën.
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Vergif.
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Wetenschappelijke technieken.
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| Dewey Classification
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579.3/165
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| LC Classification
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QP632.B3B334 2000
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| NLM classification
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W1ME9616J v.145 2000
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QW 25B131 2000
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44.43bcl
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HE 41blsrissc
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| Added Entry
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Holst, Otto.
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