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" LC-MS/MS in proteomics : "
edited by Pedro R. Cutillas, John F. Timms.
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BL
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| Record Number
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999102
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b753472
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| Title & Author
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LC-MS/MS in proteomics : : methods and applications /\ edited by Pedro R. Cutillas, John F. Timms.
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| Publication Statement
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New York, NY :: Humana Press,, ©2010.
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| Series Statement
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Methods in molecular biology,; 658
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Springer protocols
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1 online resource (x, 357 pages) :: illustrations
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| ISBN
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1607617803
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: 9781607617808
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160761779X
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9781607617792
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Includes bibliographical references and index.
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| Contents
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Approaches and applications of quantitative LC-MS for proteomics and activitomics / Pedro R. Cutillas and John F. Timms -- Overview of quantitative LC-MS techniques for proteomics and activitomics / John F. Timms and Pedro R. Cutillas -- Instrumentation for LC-MS/MS in proteomics / Robert Chalkley -- Bioinformatics for LC-MS/MS-based proteomics / Richard J. Jacob -- Analysis of post-translational modifications by LC-MS/MS / Hannah Johnson and Claire E. Eyers -- In-depth analysis of protein phosphorylation by multidimensional ion exchange chromatography and mass spectrometry / Maria P. Alcolea and Pedro R. Cutillas -- Mapping of phosphorylation sites by LC-MS/MS / Bertran Gerrits and Bernd Bodenmiller -- Analysis of carbohydrates on proteins by offline normal-phase liquid chromatography MALDI-TOF-MS/MS / Theodora Tryfona and Elaine Stephens -- Selected reaction monitoring applied to quantitative proteomics / Reiko Kiyonami and Bruno Domon -- Basic design of MRM assays for peptide quantification / Andrew James and Claus Jorgensen -- Proteome-wide quantitation by SILAC / Kristoffer T.G. Rigbolt and Blagoy Blagoev -- Quantification of proteins by iTRAQ / Richard D. Unwin -- Quantification of proteins by label-free LC-MS/MS / Yishai Levin and Sabine Bahn -- Protocol for quantitative proteomics of cellular membranes and membrane rafts / Andrew J. Thompson and Ritchie Williamson -- Organelle proteomics by label-free and SILAC-based protein correlation profiling / Joern Dengjel, Lis Jakobsen, and Jens S. Andersen -- Mapping protein-protein interactions by quantitative proteomics / Joern Dengjel, Irina Kratchmarova, and Blagoy Blagoev -- Analysis of serum proteins by LC-MS/MS / Sarah Tonack, John P. Neoptolemos, and Eithne Costello -- Urinary proteome profiling using 2D-DIGE and LC-MS/MS / Mark E. Weeks -- Analysis of peptides in biological fluids by LC-MS/MS / Pedro R. Cutillas -- Quantification of protein kinase activities by LC-MS / Maria P. Alcolea and Pedro R. Cutillas -- A protocol for top-down proteomics using HPLC and ETD/PTR-MS / Sarah R. Hart.
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| Abstract
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With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.
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Liquid chromatography.
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Proteomics.
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Tandem mass spectrometry.
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Animal Biochemistry.
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Health Biological Sciences.
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Human Anatomy Physiology.
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Liquid chromatography.
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Proteomics.
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Tandem mass spectrometry.
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Chromatography, Liquid-- methods.
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Proteomics-- methods.
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Tandem Mass Spectrometry-- methods.
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Computational Biology-- methods.
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| Dewey Classification
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572/.6
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| LC Classification
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QP551.L36 2010
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| NLM classification
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QU 58.5L432 2010
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| Added Entry
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Cutillas, Pedro R.
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Timms, John F., (John Francis)
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| Parallel Title
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Liquid chromatography with tandem mass spectrometry in proteomics
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